R26-CAG-2xKRAB-dCas9-IRES-EGFP

모델명

C57BL/6JSmo-Gt(ROSA)26Sorem1(CAG-Loxp-2xKRAB-dCas9-IRES-EGFP)Smoc

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NM-KI-18007

모델 상태

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유전자 요약

Gene Symbol
Gt(ROSA)26Sor

모델 설명

These mice harbor a CAG-LSL-2xKRAB-dCas9-IRES-EGFP-WPRE-pA conditional expression cassette in mouse Rosa26 loci via homologous recombination. This strain can be used in CRISPR mediated target gene transcriptional inhibition (CRISPRi). The loxp-stop-loxp cassette was deleted.
신청분야:CRISPRi,gene regulation tool

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Validation of 2xKRAB-dCas9 expression by  Western blot

Rosa26- CAG-LSL-2xKRAB-dCas9 mice were crossed with Dppa3-Cre mice to delete STOP cassette.

Anti-Cas9 (1).jpg

Validation of EGFP expression by fluorescence assay

CRISPRi—inhibitor: dCas9-KRAB_06.png


당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

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Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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