Apoe-KO(2)/hANGPTL3(4)
모델명
C57BL/6JSmo-Angptl3tm1(hANGPTL3)Apoeem5Smoc
카탈로그
NM-XA-242593
모델 상태
Repository Live
모델 설명
검증 데이터
Fig1. The body weight of male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 for each group). There were no significant differences in body weight or body weight gain between Apoe-KO(2)/hANGPTL3(4) mice and WT mice.
Abbr. WT, wild-type; HO, homozygous.
Fig2. Blood biochemical test of hepatic function in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions. There were no significant differences in ALT or AST levels between Apoe-KO(2)/hANGPTL3(4) mice and WT mice, with both markers varying within a similar range between the two groups. Serum samples were collected following a 6-hour fast to measure levels of (A) aspartate aminotransferase (AST), (B) alanine aminotransferase (ALT) (n=3 for in each group, Mean ± SEM, T-test).
Abbr. WT, wild-type; HO, homozygous.
Fig3. Blood lipid profile of male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions. Apoe-KO(2)/hANGPTL3(4) mice exhibited significantly elevated serum TC and LDL-C levels compared to WT controls, and showed trends toward to higher TG levels and lower HDL-C levels, indicating lipid dysmetabolism in Apoe-KO(2)/hANGPTL3(4) mice. Serum samples were collected following a 6-hour fast to measure levels of (A) triglycerides (TG), (B) total cholesterol (TC), (C) low-density lipoprotein cholesterol (LDL-C), and (D) high-density lipoprotein cholesterol (HDL-C). (n=3 for in each group, Mean ± SEM, T-test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
Abbr. WT, wild-type; HO, homozygous.
Fig4. Detection of serum hANGPTL3 protein levels in Apoe-KO(2)/hANGPTL(4) mice under chow diet conditions. hANGPTL3 was detectable exclusively in homozygous Apoe-KO(2)/hANGPTL3(4) mice but not in WT mice. Serum hANGPTL3 levels in Apoe-KO(2)/hANGPTL3(4) mice were comparable across different ages. Serum samples were collected following a 6-hour fast to measure hANGPTL3 levels using an ELISA kit (n=3 in each group, Mean ± SEM).
Abbr. WT, wild-type; HO, homozygous.
Fig5. Oil Red O staining of aortic valves in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 in each group, 20 weeks old). No lipid accumulation was observed in the aortic valves of WT mice at 5 months of age. In contrast, all Apoe-KO(2)/hANGPTL3(4) mice exhibited significant lipid accumulation in aortic valves at 5 months of age.
Abbr. WT, wild-type; HO, homozygous.
Fig6. Oil Red O staining of aortas in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 in each group, 20 weeks old). No lipid accumulation was observed in aortas of WT mice at 5 months of age. In contrast, Apoe-KO(2)/hANGPTL3(4) mice exhibited significant lipid accumulation near the aortic arch at 5 months of age.
Abbr. WT, wild-type; HO, homozygous.
당신은 또한 좋아할 수 있습니다
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
조회Milestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
조회