hMAPT
모델명
C57BL/6JSmo-Mapttm1(hMAPT)Smoc
카탈로그
NM-HU-200116
모델 상태
Repository Live
유전자 요약
Gene Symbol
Mapt
모델 설명
검증 데이터
Fig.1 Detection of MAPT expression in cerebellum by RT-PCR.
Wild type: only one band at 200 bp with primers F1/R1 (mMapt);
Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT);
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of MAPT expression in cerebellum by RT-PCR. Cerebellum RNA was extracted from 11-12-week-old male wild-type C57BL/6 (WT)(n=3) and 11-14-week-old male homozygous hMAPT knockin mice (HO) (11w: n=2, 12w: n=3, 13w: n=2, 14w: n=1), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Human MAPT mRNA (2079 bp) was detectable only in homozygous hMAPT knockin mice but not in wild-type mice.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.3 Detection of MAPT expression in cerebellum by qPCR. Cerebellum RNA was extracted from 11-12-week-old male wild-type C57BL/6 (WT)(n=3) and 11-14-week-old male homozygous hMAPT knockin mice (HO) (11w: n=2, 12w: n=3, 13w: n=2, 14w: n=1), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with human MAPT and mouse Gapdh primers. Relative expression represents the human MAPT mRNA level relative to its average expression in homozygous hMAPT knockin mice. Human MAPT mRNA was detectable only in homozygous hMAPT knockin mice but not in wild-type mice.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.4 Detection of MAPT expression in cerebellum by qPCR. Cerebellum RNA was extracted from 11-12-week-old male wild-type C57BL/6 (WT)(n=3) and 11-14-week-old male homozygous hMAPT knockin mice (HO) (11w: n=2, 12w: n=3, 13w: n=2, 14w: n=1), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Mapt and mouse Gapdh primers. Relative expression represents the mouse Mapt mRNA level relative to its average expression in homozygous hMAPT knockin mice. Mouse Mapt mRNA was detectable only in wild-type mice but not in homozygous hMAPT knockin mice.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig 5. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC.
Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.
Abbr. HO/HO, homozygous; +/+, wild type.
Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.
Fig 6. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC.
Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody.
Abbr. HO/HO, homozygous; +/+, wild type.
Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.
Fig 7. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF.
Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody.
Abbr. HO/HO, homozygous; +/+, wild type.
Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.
당신은 또한 좋아할 수 있습니다
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
조회Milestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
조회