Mefv-hMEFV*V726A
모델명
C57BL/6JSmo-Mefvtm1(hMEFV-V726A)Smoc
카탈로그
NM-KI-200221
모델 상태
Sperm cryopreservation
유전자 요약
Gene Symbol
Mefv
모델 설명
검증 데이터
Fig.1 Detection of MEFV expression in bone marrow, spleen and lung by RT-PCR. Human MEFV mRNA (421 bp) was detectable only in heterozygous Mefv-hMEFV*V726A knockin mice but not in wild-type mice. Bone marrow, spleen and lung RNA was extracted from 4-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous Mefv-hMEFV*V726A knockin mice (HE) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HE, heterozygous; WT, wild type.
Fig.2 mRNA sequencing of Mefv-hMEFV*V726A mice. Sequencing data of RT-PCR products confirmed the presence of target mutations in Mefv-hMEFV*V726A mice.
Abbr. HE, heterozygous; WT, wild type.
Fig.3 Detection of MEFV expression in Mefv-hMEFV*V726A mice by WB. MEFV was detectable in spleen, bone marrow and lung from both WT C57BL/6 and heterozygous Mefv-hMEFV*V726A mice, as the antibody was cross-reactived between human and mouse MEFV. The spleen, bone marrow and lung tissue lysates were collected from 4-week-old male wild-type C57BL/6 mice and 4-week-old male heterozygous Mefv-hMEFV*V726A mice, and then analyzed by western blot with anti-Mefv antibody.
Abbr. HE, heterozygous; WT, wild type.
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
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Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
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