hIDO1
모델명
B6;129S-Ido1tm1(hIDO1)Smoc
카탈로그
NM-HU-00061
모델 상태
Repository Live
유전자 요약
Gene Symbol
IDO1
모델 설명
검증 데이터
Fig.1 Detection of IDO1 expression in the thymus of WT C57BL/6 mice (male, 8-10 weeks old) and hIDO1 knockin mice (male, 9 weeks old) by RT-PCR.
Wild type: one band at 165 bp with primers F1/R1(mIdo1);
Homozygous: only one band at 112 bp with primers F2/R2(hIDO1).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human IDO1 expression in WT C57BL/6 mice (male, 20 weeks old) and hIDO1 knockin mice (male, 19 weeks old) by WB.
Abbr. M, marker; WT, wild type; HO, Homozygous.
Note. Mice were injected intraperitoneally with mouse IFN-γ (1.25 mg/kg) and sacrificed at 4 h or 48 h post-injection. Arrow indicates expected molecular weight and asterisk indicates a nonspecific band.
Fig.3 Detection of human IDO1 expression in splenocytes from WT C57BL/6 mice (female, 8 weeks old) and hIDO1 knockin mice (female, 24 weeks old) by WB.
Abbr. M, marker; WT, wild type; HO, Homozygous; mIFNγ: mouse IFNγ.
Note. “+ “and “−” indicate that splenocytes were cultured with or without mouse IFN-γ (50 ng/mL) for 16 h in vitro, respectively.
당신은 또한 좋아할 수 있습니다
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
조회Milestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
조회