hIL2RA/hIL2RB/hIL2RG
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C57BL/6JSmo-Il2ratm1(hIL2RA)Il2rgtm1(IL2RG)Il2rbtm1(hIL2RB)Smoc
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NM-HU-210414
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Repository Live
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Fig1. Detection of IL2RA expression in thymus and spleen by RT-PCR.
Wild type: only one band at 350 bp with primers F1/R1(mIl2ra);
Homozygous: only one band at 318 bp with primers F2/R2(hIL2RA);
Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig2. Detection of IL2RB expression in thymus and spleen by RT-PCR.
Wild type: only one band at 360 bp with primers F1/R1(mIl2rb);
Homozygous: only one band at 281 bp with primers F2/R2(hIL2RB);
Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig3. Detection of IL2RG expression in thymus and spleen by RT-PCR.
Wild type: only one band at 400 bp with primers F1/R1(mIl2rg);
Homozygous: only one band at 263 bp with primers F2/R2(hIL2RG);
Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.
Fig.4 Human IL-2 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old).
Splenocytes were collected from the spleen, treated with different concentrations of human IL-2 for 15 min and analyzed by flow cytometry. As shown in the figure, human IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.
Fig.5 Mouse IL-2 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old).
Splenocytes were collected from the spleen, treated with different concentrations of mouse IL-2 for 15 min and analyzed by flow cytometry. As shown in the figure, mouse IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg. However, the activation of mouse IL-2 is weaker than that of human IL-2 in hIL2RA/hIL2RB/hIL2RG knockin mice and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice.
Fig.6 Human IL-15 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old).
Splenocytes were collected from the spleen, treated with different concentrations of human IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-15 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.
Fig.7 Mouse IL-15 induced the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, NK cells and Treg in the C57BL/6 WT mouse (n=2, 6 weeks old), hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 14 weeks old) and hIL2/hIL2RA/hIL2RB/hIL2RG knockin mice (n=2, 17 weeks old).
Splenocytes were collected from the spleen, treated with different concentrations of human IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-15 induced pSTAT5 in CD4+ T cells, CD8+ T cells, NK cells, and Treg.
Fig.8 Analysis of the phosphorylation of Stat5 in CD4+ T cells, CD8+ T cells, and Treg in human PBMC.
PBMC were treated with different concentrations of human IL-2, human IL-15, mouse IL-2, and mouse IL-15 for 15 min, and analyzed by flow cytometry. As shown in the figure, human IL-2, human IL-15, and mouse IL-2 induced pSTAT5 in CD4+ T cells, CD8+ T cells, and Treg in a dose dependent manner. However, the activation of mouse IL-2 is weaker than that of human IL-2 and human IL-15.
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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