Cdkn2a-2A-DreERT2(p16-2A-DreERT2)
모델명
C57BL/6JSmo-Cdkn2aem(2A-DreERT2)1Smoc
카탈로그
NM-KI-225086
모델 상태
Repository Live
유전자 요약
Gene Symbol
Cdkn2a
모델 설명
검증 데이터
Fig.1 Detection of Cdkn2a expression in kidney, lung, liver and skin by qPCR. Cdkn2a mRNA was detected at comparable levels and exhibited similar expression profiles in both wild-type and heterozygous Cdkn2a-2A-DreERT2 knockin mice, indicating that the knockin allele did not disrupt the endogenous expression pattern of the gene. Kidney, lung, liver and skin RNA was extracted from 9-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous Cdkn2a-2A-DreERT2 knockin mice (HO)(n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Cdkn2a(F3/R3) and mouse Gapdh primers. Relative expression represents the mouse Cdkn2a(F3/R3) mRNA level relative to heterozygous Cdkn2a-2A-DreERT2 knockin mice expression in skin .
Abbr. M, marker; HE, heterozygous; WT, wild type.
Fig.2 Detection of DreERT2 expression in kidney, lung, liver and skin by qPCR. DreERT2 mRNA was detectable in heterozygous Cdkn2a-2A-DreERT2 knockin mice but not in wild-type mice. Kidney, lung, liver and skin RNA was extracted from 9-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous Cdkn2a-2A-DreERT2 knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with DreERT2 and mouse Gapdh primers. Relative expression represents the DreERT2 mRNA level relative to heterozygous Cdkn2a-2A-DreERT2 knockin mice expression in skin.
Abbr. M, marker; HE, heterozygous; WT, wild type.
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
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Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
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