hIL2

모델명

C57BL/6JSmo-Il2tm3(hIL2)Smoc

카탈로그

NM-HU-190048

모델 상태

Embryo cryopreservation

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유전자 요약

Gene Symbol
IL2

모델 설명

The endogenous mouse Il2 gene was replaced by human IL2 gene.
신청분야:Immunotherapy,cancer research,drug screening

검증 데이터

image.png

Fig1. Detection of IL2 expression by RT-PCR. 

Wild type: only one band at 255 bp with primers F2/R2(mIl2); Homozygous: only one band at 320 bp with primers F1/R1(hIL2). 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

IL2-Hu.png

Fig2. Expression of human IL2 in the Serum of IL2-HU heterozygous mice is detected by ELISA.

IL2-Mu.png


Fig3. Expression of murine IL2 in the Serum of IL2-HU heterozygous mice and  is detected by ELISA.

IL2-3.png

Fig4. Splenocytes from humanized IL2 mice were analyzed by flow cytometry after activation. 

hIL2+ (but not mIL2+) cells were detectable in the homozygous hPD-L2 mice. 


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