hCD73(2)

모델명

C57BL/6Smoc-Nt5etm3(NT5E)Smoc

카탈로그

NM-HU-200009

모델 상태

Embryo cryopreservation

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유전자 요약

Official Symbol
NT5E

모델 설명

The endogenous mouse Nt5e gene was replaced by human NT5E gene.
신청분야:Immunotherapy,cancer research,drug screening

검증 데이터

image.png

Fig1. Detection of NT5E expression in liver and lung by RT-PCR. Wild type: only one band at 221 bp with primers F1/R1(mNt5e);Homozygous: only one band at 285 bp with primers F2/R2(hNT5E); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

cd73-1.png

Fig2. Expression of CD73 in the  peripheral T cells collected from humanized CD73 mice were detected by FACS. The results showed that the expression of human CD73 can be detected in the  peripheral T cells of humanized CD73 mice as same as in Hu-PBMC.

cd73-2.png

Fig.3 In vivo validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized CD73 mice. Homozygous humanized CD73 mice were inoculated with MC38 colon cancer cells (expressing human CD73 rather than murine CD73). Mice were grouped when the tumor size was approximately 100 mm3(n=6). Three human CD73 antibodies differently inhibited tumor growth, confirming that the CD73-HU mouse model is a powerful tool for in vivo CD73 antibody pharmacological efficacy study. A.Tumor average volume ± SEM, B.Mice average weight ± SEM.


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