Cd8a-IRES-DTRGFP

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C57BL/6Smoc-Cd8aem2(IRES-DTREGFP)Smoc

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NM-KI-190045

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Cd8a

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A IRES-DTREGFP expression cassette was knocked into the Cd8a gene stop codon site.
신청분야:DTR mouse

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EGFP expression

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Fig1.  Validation EGFP expression in PBMC by FACS.(Cooperation with CrownBio)

Validation data of DTR function

Heterozygous CD8a-IRES-DTRGFP mice and C57BL/6 mice were inoculated with MC38 colon cancer cells. After the tumors grew to 100 mm3, the CD8a-IRES-DTRGFP mice were injected with Diphtheria toxin. 

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Fig2. The growth of MC38 tumors in Heterozygous CD8a-IRES-DTRGFP mice and C57BL/6 mice. (Cooperation with CrownBio)

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Fig3. The percent of CD8+ T cell in peripheral blood of MC38 tumor-bearing CD8a-IRES-DTRGFP mice and C57BL/6 mice is detected by FACS. (Cooperation with CrownBio)

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Fig4. The percent of CD8+ T cell in peripheral blood of MC38 tumor-bearing CD8a-IRES-DTRGFP mice and C57BL/6 mice is detected by FACS. Nearly complete depletion was  achieved only after 1 dose administration of DT and the depletion effect  lasted to day 14.(Cooperation with CrownBio)


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