hCTLA-4

모델명

C57BL/6Smoc-Ctla4em1(hCTLA4) Smoc

카탈로그

NM-HU-00014

모델 상태

Embryo cryopreservation

PDF 받기

유전자 요약

Official Symbol
CTLA4

모델 설명

The endogenous mouse Ctal4 gene was replaced by human CTLA4 gene. While hCTLA4(2)(Stock No.NM-HU-190038) mice function similarly to hCTLA4 mice,for more detailed information please contact our technical advisor.
신청분야:cancer research,Immunotherapy,drug screening;

검증 데이터

  • Flow cytometry (FACS) analysis data of humanized CTLA4 mouse

免疫检查点人源化小鼠-18.05.16-002-01.jpg

Figure 1. Expression of CTLA4 in the activated spleen lymphocytes of humanized CTLA4 mice is detected by FACS. The spleen lymphocytes of homozygous humanized CTLA4 mice were activated by anti-CD3 and anti-CD28 for 72 hours, and then collected for staining. The expression of humanized CTLA4 was detected by FACS. The results showed that the active expression of humanized CTLA4 can be detected in both activated CD4+ and CD8+ T lymphocytes collected from homozygous humanized CTLA4 mice. (Completed in collaboration with CrownBio).

  • In vivo validation in a MC38 tumor-bearing model of humanized CTLA4 mouse

CTLA4-1.png

CTLA4-2.png

Figure 2. In vivo anti-tumor effect of Ipilimumab, a humanized anti-CTLA4 antibody, in a humanized mouse model of CTLA4

In vivo validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized CTLA4 mice. Homozygous humanized CTLA4 mice were inoculated with MC38 colon cancer cells. The results showed: Yervoy, a drug targeting human CTLA4, showed a very significant anti-tumor effect (p<0.001), demonstrating that the humanized CTLA4 mice are a good in vivo model for validating the efficacy of antibodies targeting human CTLA4.


당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

조회
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

조회