hIL13(BALB/c)

모델명

BALB/cAnSmoc-Il13em2(hIL13)Smoc

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NM-HU-220019

모델 상태

Embryo cryopreservation

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유전자 요약

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IL13

모델 설명

The endogenous mouse Il13 gene was replaced by human IL13 gene.

검증 데이터

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Fig1. Detection of IL-13 expression in thymus by RT-PCR. Wild type: only one band at 260 bp with primers F1/R1(mIl-13);Heterozygous: one band at 260 bp with primers F1/R1(mIl-13) and one band at 334 bp with primers F2/R2(hIL-13); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hIL13 expression in cell culture supernatant by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. Splenocytes from BALB/c and homozygous hIL13 mice were stimulated with ConA in vitro.

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Fig3. Detection of mIL13 expression in cell culture supernatant by ELISA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type. Note. Splenocytes from BALB/c and homozygous hIL13 mice were stimulated with ConA in vitro.


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