hADORA2A

모델명

C57BL/6Smoc-Adora2atm1(hADORA2A)Smoc

카탈로그

NM-HU-215061

모델 상태

Embryo cryopreservation

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유전자 요약

Official Symbol
Adora2a

Synonyms

A2aR; A2AAR; AA2AR

NCBI ID

11540

MGI ID

99402

Ensembl ID

ENSMUSG00000020178

Pubmed

Adora2a

Human Ortholog

ADORA2A

모델 설명

The endogenous mouse Adora2a gene was replaced by human ADORA2A gene.

검증 데이터

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Fig1. Detection of ADORA2A expression in thymus and spleen by RT-PCR. Wild type: only one band at 184 bp with primers F1/R1 (mAdora2a); Homozygous: only one band at 234 bp with primers F2/R2 (hADORA2A); Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hADORA2A expression in Myeloid cells and activated T cells in hADORA2A KI mice. Note. Splenocytes from C57BL/6 and homozygous hADORA2A mice were stimulated with anti-mCD3 antibody in vitro. The anti-human ADORA2A antibody cross-reacted with mADORA2A and humanized ADORA2A.

image.png

Fig3. Detection of hADORA2A expression in activated B cells and NK cells in hADORA2A KI mice. Note. Splenocytes from C57BL/6 and homozygous hADORA2A mice were stimulated with anti-mCD3 antibody in vitro. The anti-human ADORA2A antibody cross-reacted with mADORA2A and humanized ADORA2A.

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Fig4. Detection of mA2AR expression in hippocampus(CA3) in C57BL/6 mice by IHC. Abbr. HO/HO, homozygous; +/+, wild type. Note. The anti-mouse ADORA2A Antibody cross-reacted with mADORA2A and humanized ADORA2A.

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Fig5. Detection of hA2AR expression in hippocampus(CA3) in HO hADORA2A KI mice by IHC. Abbr. HO/HO, homozygous; +/+, wild type. Note. The anti-human ADORA2A Antibody cross-reacted with mADORA2A and humanized ADORA2A.

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