Cdh5-2A-CreERT2

모델명

C57BL/6Smoc-Cdh5em1(2A-CreERT2-WPRE-polyA)Smoc

카탈로그

NM-KI-200173

모델 상태

Repository Live

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유전자 요약

Official Symbol
Cdh5

Synonyms

7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225

NCBI ID

12562

MGI ID

105057

Ensembl ID

ENSMUSG00000031871

Pubmed

Cdh5

Human Ortholog

CDH5

모델 설명

A 2A-CreERT2-WPRE-polyA expression cassette was knocked into the Cdh5 gene stop codon site.
신청분야：CDH5(vascular endothelial cadherin ,VEC) is expressed in the vasculature. VEC-Cre will mark both endothelial cells and blood cells in lineage tracing experiments.

검증 데이터

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Fig. 1 CreERT2-mediated recombination in the aorta of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the arterial endothelial cells of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

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Fig. 2 CreERT2-mediated recombination in the spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the endothelial cells derived from spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

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Fig. 3 CreERT2-mediated recombination in the cerebellum of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in some cells of cerebellum derived from Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.


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Fig. 4 Detection of tdTomato(red) in various tissues of Cdh5CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the brain, pituitary gland, eyes, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle, intestine, colon, spleen, thymus and brown fat. TdTomato expression can not be observed in the epidermis. (For more detailed information please contact our technical advisor.)


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