Lgr5-EGFP-IRES-CreERT2
모델명
C57BL/6Smoc-Lgr5em1(EGFP-IRES-creERT2-WPRE-polyA)Smoc
카탈로그
NM-KI-200154
모델 상태
Repository Live
유전자 요약
Official Symbol
Lgr5
모델 설명
검증 데이터
Fig. 1 CreERT2-mediated recombination in the stomach of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the gastric mucosa and gastric crypt of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.
Fig. 2 CreERT2-mediated recombination in the small intestine of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of small intestine derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.
Fig. 3 CreERT2-mediated recombination in the large intestine of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of large intestine derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.
Fig. 4 CreERT2-mediated recombination in the hair follicles of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the hair follicles and individual cells of subcutaneous tissue derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.
Fig. 5 Detection of tdTomato(red) and GFP(green) in various tissues of Lgr5CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the glandular stomach, large intestine, small intestine and hair follicles. Some leakiness were detected prior to tamoxifen exposure. TdTomato expression can not be observed in the brain, pituitary gland, thymus, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle and brown fat. EGFP expression can not be observed.(For more detailed information please contact our technical advisor.)
Fig. 6 The EGFP protein expression was detected in the crypt cells of intestine by immunofluorescence staining using anti-EGFP antibody.
당신은 또한 좋아할 수 있습니다
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
조회