Lgr5-EGFP-IRES-CreERT2

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C57BL/6Smoc-Lgr5em1(EGFP-IRES-creERT2-WPRE-polyA)Smoc

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NM-KI-200154

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Lgr5

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A EGFP-IRES-creERT2-WPRE-polyA expression cassette was knocked into the Lgr5 gene start codon site.
신청분야:Cre recombinase tool; Lgr5 marks stem cells in the stomach, small intestine, colon, and hair follicle, including  gastric epithelial stem cells, intestinal crypt cells and hair follicle stem cells.

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Fig. 1 CreERT2-mediated recombination in the stomach of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the gastric mucosa and gastric crypt of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.

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Fig. 2 CreERT2-mediated recombination in the small intestine of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of small intestine derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.


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Fig. 3 CreERT2-mediated recombination in the large intestine of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the mucous membrane epithelial cells and crypt cells of large intestine derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.


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Fig. 4 CreERT2-mediated recombination in the hair follicles of Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the hair follicles and individual cells of subcutaneous tissue derived from Lgr5CreERT2 /+; Rosa26tdTomato/+ mouse. EGFP(green) expression can not be observed.

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Fig. 5 Detection of tdTomato(red) and GFP(green) in various tissues of Lgr5CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the glandular stomach, large intestine, small intestine and hair follicles. Some leakiness were detected prior to tamoxifen exposure. TdTomato expression can not be observed in the brain, pituitary gland, thymus, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle and brown fat. EGFP expression can not be observed.(For more detailed information please contact our technical advisor.)

NM-KI-200154;Lgr5-EGFP-IRES-CreERT2-1.png

Fig. 6 The EGFP protein expression was detected in the crypt cells of intestine by immunofluorescence staining using anti-EGFP antibody.


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