Nos1-CreERT2

모델명

C57BL/6Smoc-Nos1em1(CreERT2-SV40polyA)Smoc

카탈로그

NM-KI-200091

모델 상태

Sperm cryopreservation

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유전자 요약

Official Symbol
Nos1

Synonyms

NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik

NCBI ID

18125

MGI ID

97360

Ensembl ID

ENSMUSG00000029361

Pubmed

Nos1

Human Ortholog

NOS1

모델 설명

A CreERT2-SV40polyA expression cassette was knocked into the Nos1 gene start codon site.
신청분야：Nos1, the neurogenic nitric oxide synthase, is a key enzyme responsible for the production of the signaling molecule nitric oxide (NO) by neurons. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre after tamoxifen treatment.

검증 데이터

NM-KI-200091；Nos1-CreERT2-1.png

Fig. 1 CreERT2-mediated recombination in the brian of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the NOS1 positive neurons of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091；Nos1-CreERT2-2.png

Fig. 2 CreERT2-mediated recombination in the large intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the large intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091；Nos1-CreERT2-3.png

Fig. 3 CreERT2-mediated recombination in the small intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the small intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200091；Nos1-CreERT2-4.png

Fig. 4 Detection of tdTomato(red) in various tissues of Nos1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in some cells of the brain and intestine. Some leakiness were detected prior to tamoxifen exposure. Besides, tamoxifen-dependent cre recombinase expression can also be detected in some cells derived from eyes, lung, submandibular gland, kidney, spleen, thymus, muscle and ovary, except for heart, brown fat tissue and liver. (For more detailed information please contact our technical advisor.)

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