H11-pAlb-hBAFF

Fig.1 Detection of hBAFF expression in serum by ELISA. 

Abbr.  WT, wild type; HE, heterozygous.

Fig.2 Detection of immune phenotype in WT C57BL/6 and HE H11-pAlb-hBAFF mice.

Fig.3 Detection of phenotype in Peripheral blood in WT C57BL/6 and HE H11-pAlb-hBAFF mice.

Fig.4 Detection of phenotype in Spleen in WT C57BL/6 and HE H11-pAlb-hBAFF mice.

Fig.5 Detection of phenotype in Lymph node in WT C57BL/6 and HE H11-pAlb-hBAFF mice.

Fig.6 Detection of phenotype in Bone marrow in WT C57BL/6 and HE H11-pAlb-hBAFF mice.

Fig.7 Body weight of H11-pAlb-hBAFF mice at different time points. 

Fig.8 Tissue weights and photos of H11-pAlb-hBAFF mice at different time points.

Enlarged spleen and Peyer's patches in H11-pAlb-hBAFF mice.

Fig.9 Detect the dsDNA levels in the blood of H11-pAlb-hBAFF mice. (n=5~7/group, Kit information: Apha Diagnostic, 5120)

Fig.10 Expression characterization of mIgA (A), mIgE (B), mIgM (C) and mIgG (D) in HE H11-pAlb-hBAFF mice by ELISA.

Fig.11 Urinary Biochemical Analysis of H11-pAlb-hBAFF mice (n=5~7/group).

Fig.12 H&E Staining and pathological analysis results of Kidney.

Green arrow: mild edema of renal tubular epithelial cells; Blue arrow: mild vacuolar degeneration of renal tubular epithelial cells; Light-green arrow: frequent mesangial matrix proliferation in glomeruli.

Fig.13 HE staining in the kidneys of 27-week-old WT C57BL/6 and heterozygous H11-pAlb-hBAFF mice. 

The results demonstrated that, compared with C57BL/6 mice, H11-pAlb-hBAFF mice exhibited significant pathological changes in the kidneys (27 weeks old, n=3-4). Scale bar, 200 μm.

Fig.14 Immunohistochemistry staining of IgG deposition in the kidneys of WT C57BL/6 and heterozygous H11-pAlb-hBAFF mice. Kidneys tissues were collected from mice and subjected to IHC staining using an anti-mouse IgG antibody. The results showed that, compared with C57BL/6 mice, H11-pAlb-hBAFF mice exhibited remarkable IgG deposition in the kidneys (27 weeks old, n=3-4). IgG deposition was shown by a brown staining. Each image represents a mouse. Scale bar, 50 μm.

Fig.15 Representative images of  IgA immunofluorescence staining in the kidneys of WT C57BL/6 and heterozygous H11-pAlb-hBAFF mice. Kidneys tissues were collected from mice and subjected to IgA immunofluorescence staining using an anti-mouse IgA antibody. IgA deposition was shown by a red staining. Mean ± SEM, Unpaired t-test, **P<0.01. Scale bar, 20 μm. Immunofluorescence staining for IgA revealed conspicuous granular deposits in the glomerular mesangial areas and along renal tubules of H11-pAlb-hBAFF mice (27 weeks old, n=3-4), compared with WT C57BL/6 mice.