hB4GALNT1
모델명
C57BL/6JSmo-B4galnt1em1(hB4GALNT1)Smoc
카탈로그
NM-HU-220337
모델 상태
Repository Live
유전자 요약
Gene Symbol
B4galnt1
모델 설명
검증 데이터
Fig.1 Detection of B4GALNT1 expression in duodenum by RT-PCR. Duodenum RNA was extracted from 12-year-old male wild-type C57BL/6 (WT)(n=3) and homozygous hB4GALNT1 knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse B4galnt1 mRNA (261 bp) was detectable only in wild-type C57BL/6 mice. Human B4GALNT1 mRNA (235 bp) was detectable only in homozygous hB4GALNT1 knockin mice but not in wild-type mice.
Fig.2 Detection of B4GALNT1 expression in hB4GALNT1 mice by WB. B4GALNT1 was detectable in hippocampus from both WT C57BL/6 and HO hB4GALNT1 mice , as the antibody was cross-reactived between human and mouse B4GALNT1. The hippocampus tissue lysates were collected from 11-week-old WT C57BL/6 mice and 13-week-old male HO hB4GALNT1 mice, and then analyzed by western blot with anti-B4GALNT1 antibody.
Abbr. HO, homozygous; WT, wild type; PC1, positive control, hela cells; PC2, positive control, NIH-3T3 cells.
당신은 또한 좋아할 수 있습니다
Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
조회Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!
At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
조회Milestones
Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.
조회