Lep-KO

모델명

B6;129S-Leptm1Smoc

카탈로그

NM-KO-00034

모델 상태

Repository Live

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유전자 요약

Official Symbol
Lep

Synonyms

ob; obese

NCBI ID

16846

MGI ID

104663

Ensembl ID

ENSMUSG00000059201

Pubmed

Lep

Human Ortholog

LEP

모델 설명

Exon 2 was replaced by Neo cassette.
신청분야：Insulin resistance,obesity,and type 2 diabetes etc.

Disease Connection

Abdominal Obesity-Metabolic Syndrome

Phenotype(s)

MGI:4429407

Reference(s)

Xu A, Liu J, Liu P, Jia M, Wang H, Tao L, Mitochondrial translocation of Nur77 induced by ROS contributed to cardiomyocyte apoptosis in metabolic syndrome. Biochem Biophys Res Commun. 2014 Apr 18;446(4):1184-9

Abdominal Obesity-Metabolic Syndrome 1

Phenotype(s)

MGI:2654709

Reference(s)

Ikels K, Kuschel S, Fischer J, Kaisers W, Eberhard D, Ruther U, FTO is a relevant factor for the development of the metabolic syndrome in mice. PLoS One. 2014;9(8):e105349

Non-Alcoholic Fatty Liver Disease

Phenotype(s)

MGI:5807153

Reference(s)

Trak-Smayra V, Paradis V, Massart J, Nasser S, Jebara V, Fromenty B, Pathology of the liver in obese and diabetic ob/ob and db/db mice fed a standard or high-calorie diet. Int J Exp Pathol. 2011 Dec;92(6):413-21

Obesity

Phenotype(s)

MGI:3623749

Reference(s)

Barouch LA, Berkowitz DE, Harrison RW, O'Donnell CP, Hare JM, Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice. Circulation. 2003 Aug 12;108(6):754-9

Type 2 Diabetes Mellitus

Phenotype(s)

MGI:5428893

Reference(s)

Clee SM, Nadler ST, Attie AD, Genetic and genomic studies of the BTBR ob/ob mouse model of type 2 diabetes. Am J Ther. 2005 Nov-Dec;12(6):491-8

검증 데이터

image.png

Fig1. Body weight and Blood glucose monitoring of ob/ob mice.

image.png

Fig2. Evaluation of Oral glucose tolerance test (OGTT) of female ob/ob mice. 

Abbr. HO, homozygous; WT, wild type. **, P ≤ 0.01.

Note. The tested ob/ob and C57BL/6 mice were 6 weeks old.

image.png


Fig3. Detection of insulin levels in serum by ELISA. 

Abbr. Hom, homozygous; HE, heterozygous; WT, wild type.

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old.

image.png

Fig4. H&E staining of the subcutaneous adipose tissue (SAT) in ob/ob mice. 

Abbr. HO, homozygous; WT, wild type; WAT, white adipose tissue; BAT, brown adipose tissue. 

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 20.

image.png

Fig5. Representative pictures of pancreas in ob/ob mice. 

These results suggested the inflammatory cell infiltration of the pancreas in ob/ob mice. 

Abbr. HO, homozygous; WT, wild type. 

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 10.

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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

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